微小血管モデルによる血管微小環境の時空間解析手法の構築
【研究キーワード】
microvessel / permeability / collagen / microfluidics
【研究成果の概要】
Firstly, we have built 3D-printed mechanical devices that interface the MV (microvessel) for fluidic handling and which are compatible with optical microscopy. Two distinct experiments have been setup to probe the vessel permeability properties: i) an hydrostatic set-up for macromolecular fluorescence based permeability analysis and ii) a pressure-driven vessel deformation characterization.
Microfabrication of MV with different type of cellular models (HUVECs, HUVECs treated with inhibitor and co-culture of HUVECs with human pericytes) has been also realized and changes in permeability and mechanical reponse is currently being measured.
Moreover, we also have developed a 2D finite element model on COMSOL to simulate the poroelastic mechanical response of a microvessel embedded into a collagen matrix under a pressure stress. Preliminary results seems to show a dependence between the vessel permeability and its mechanical response, which is in accordance with experimental results.
【研究代表者】
【研究種目】特別研究員奨励費
【研究期間】2020-11-13 - 2023-03-31
【配分額】2,200千円 (直接経費: 2,200千円)